Physical and chemical aspects of the study of clusters, nanostructures and nanomaterials
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Development of a biologically active nanosystem based on riboflavin, a microelement of copper and L-lysine amino acid

A.V. Blinov1, A.B. Golik1, A.A. Gvozdenko1, E.S. Kastarnova2, V.A. Orobets2, A.A. Yakovenko1, T.N. Bakholdina1

1 North Caucasus Federal University
2 Stavropol State Agrarian University

DOI: 10.26456/pcascnn/2023.15.930

Original article

Abstract: А biologically active nanosystem based on riboflavin, copper microelement and the amino acid lysine copper lysinatoriboflavinate has been developed. The obtained sample of the biologically active additive was studied by a complex of modern methods of analysis, namely: spectrophotometry, scanning electron microscopy, spectrophotoluminescence spectroscopy. In addition, computer quantum chemical modeling was carried out. It has been established that the most energetically favorable model of a biologically active nanosystem based on riboflavin, a trace element of copper and lysine is a model in which riboflavin is bound to the copper ion through the enol oxygen at C2 and the neighboring nitrogen heteroatom N3, and lysine is bound to the copper ion through the carboxyl and amino groups in α position. As a result of the analysis of micrographs of a biologically active nanosystem based on riboflavin, copper microelement and lysine amino acid, it was found that the particles have an acicular structure, the width of individual crystals is from 100 to 300 nm, and the length reaches several microns. The energy-dispersive X-ray spectra of a biologically active nanosystem based on riboflavin, the microelement copper, and the amino acid lysine coincide with the theoretical concepts of the structure of the elements under consideration. The analysis did not show the presence of impurity compounds, which may allow us to judge the high purity of the developed compound.

Keywords: trace element copper, essential amino acids, quantum chemical modeling, vitamin B2

  • Andrey V. Blinov – Ph. D. Associate Professor, Department of Physics and Technology of Nanostructures and Materials, North Caucasus Federal University
  • Aleksey B. Golik – Assistant, Department of Physics and Technology of Nanostructures and Materials, North Caucasus Federal University
  • Aleksey A. Gvozdenko – Assistant, Department of Physics and Technology of Nanostructures and Materials, North Caucasus Federal University
  • Elena S. Kastarnova – Ph. D., Researcher, Department of Therapy and Pharmacology, Stavropol State Agrarian University
  • Vladimir A. Orobets – Dr. Sci., Professor, Department of Therapy and Pharmacology, Stavropol State Agrarian University
  • Andrey A. Yakovenko – 3rd year student, Department of Physics and Technology of Nanostructures and Materials of the Faculty of Physics and Technolog, North Caucasus Federal University
  • Tamara N. Bakholdina – 3rd year postgraduate student, Department of Food Technologies and Engineering, Faculty of Food Engineering and Biotechnology, North Caucasus Federal University

Reference:

Blinov, A.V. Development of a biologically active nanosystem based on riboflavin, a microelement of copper and L-lysine amino acid / A.V. Blinov, A.B. Golik, A.A. Gvozdenko, E.S. Kastarnova, V.A. Orobets, A.A. Yakovenko, T.N. Bakholdina // Physical and chemical aspects of the study of clusters, nanostructures and nanomaterials. — 2023. — I. 15. — P. 930-939. DOI: 10.26456/pcascnn/2023.15.930. (In Russian).

Full article (in Russian): download PDF file

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