Structural and kinetic aspects of hydroxyapatite and brushite dissolution in lactic acid
A.A. Provorkina, O.A. Golovanova
Omsk State University named after F.M. Dostoevsky
DOI: 10.26456/pcascnn/2025.17.865
Original article
Abstract: Modern dentistry and regenerative medicine face the challenge of controlled removal of pathological calcium phosphate deposits and development of biocompatible materials for tissue restoration. This study presents a comprehensive investigation of lactic acid interaction with brushite (CaHPO4ꞏ2H2O) and hydroxyapatite (Ca10(PO4)6(OH)2), which is crucial for developing dental calculus demineralization methods and creating resorbable implants. The research employed advanced analytical techniques including potentiometry with a Ca-selective electrode, X-ray phase analysis, thermogravimetric analysis, and infrared spectroscopy. Special emphasis was placed on thermodynamic modeling of dissolution processes by Gibbs free energy calculation (ΔG° = -61,8 kJ/mol). The key results demonstrated that the calcium phosphate dissolution efficiency is critically pH-dependent: at pH < 4, the protonated form of lactic acid dominates, enabling active interaction, while at pH > 8,2 the process virtually ceases. The thermal analysis revealed the formation of calcium pyrophosphate (Ca2P2O7) at 400-800°C, which is significant for bioceramic material development. These findings provide new opportunities for creating pH-controlled demineralization systems and designing biomaterials with programmable resorption rates. The study has important practical implications for dentistry (dental calculus removal) and regenerative medicine (bone implants).
Keywords: hydroxyapatite, brushite, monetite, lactic acid, degradation
- Anastasiya A. Provorkina – Lecturer, Department of Inorganic Chemistry, Omsk State University named after F.M. Dostoevsky
- Olga A. Golovanova – Dr. Sc., Professor, Head of the Department of Inorganic Chemistry, Omsk State University named after F.M. Dostoevsky
For citation:
Provorkina A.A., Golovanova O.A. Strukturno-kineticheskie aspekty rastvoreniya gidroksiapatita i brushita v molochnoj kislote [Structural and kinetic aspects of hydroxyapatite and brushite dissolution in lactic acid], Fiziko-khimicheskie aspekty izucheniya klasterov, nanostruktur i nanomaterialov [Physical and chemical aspects of the study of clusters, nanostructures and nanomaterials], 2025, issue 17, pp. 865-874. DOI: 10.26456/pcascnn/2025.17.865. ⎘
Full article (in Russian): download PDF file
References:
1. Tsolaki E., Bertazzo S. Pathological mineralization: The potential of mineralomics, Materials, 2019, vol. 12, issue 19, art. no. 3126, 22 p. DOI: 10.3390/ma12193126.
2. Miklyaev S.V., Leonova O.M., Sushchenko A.V. et al. Vliyanie razlichnykh sposobov udaleniya zubnykh otlozheniy na strukturu tkaney zuba [The influence of different methods of dental deposits removal on tooth tissue structure], Vestnik Volgogradskogo gosudarstvennogo meditsinskogo universiteta [Volgograd Medical University Bulletin], 2021, no. 3 (79), pp. 45-51. DOI: 10.19163/1994-9480-2021-3(79)-45-51. (In Russian).
3. Nakahara T., Strauss H. W. From inflammation to calcification in atherosclerosis, European journal of nuclear medicine and molecular imaging, 2017, vol. 44, issue 5, pp. 858-860. DOI: 10.1007/s00259-016-3608-x.
4. Dulaev A.K., Ovdenko A.G., Varzin S.A., Dulaev D.V. Infektsionnye oslozhneniya v travmatologii i ortopedii [Infectious complications in traumatology and orthopedics], Vestnik Sankt-Peterburgskogo universiteta. Meditsina [Bulletin of Saint Petersburg University. Medicine], 2024, vol. 19, no. 4, pp. 344-353. DOI: 10.21638/spbu11.2024.405. (In Russian).
5. Chepur S.V., Pluzhnikov N.N., Chubar O.V. Molochnaya kislota: dinamika predstavleniy o biologii laktata [Lactic acid: dynamics of ideas about lactate biology], Uspekhi sovremennoy biologii [Advances in Modern Biology], 2021, vol. 141, no. 3, pp. 227-247. DOI: 10.31857/S0042132421030042. (In Russian).
6. Volkova G.S., Kuksova E.V., Serba E.M. Izuchenie biologicheskikh mezhshtammovykh vzaimodeystviy i rostovykh svoystv proizvodstvennykh shtammov molochnokislykh bakteriy [Study of biological inter-strain interactions and growth properties of industrial lactic acid bacteria strains], Aktual'nye voprosy molochnoy promyshlennosti, mezhotraslevye tekhnologii i sistemy upravleniya kachestvom [Current issues of dairy industry, interdisciplinary technologies and quality management systems], 2020, vol. 1, no. 1, pp. 104-109. DOI: 10.37442/978-5-6043854-1-8-2020-1-104-109. (In Russian).
7. Nozari A., Rahmati A., Shamsaei Z. et. al. Destructive effects of citric acid, lactic acid and acetic acid on primary enamel microhardness, Journal of Dental School, 2015, vol. 33, issue 1, pp. 66-73. DOI: 10.22037/jds.v33i1.24751.
8. Leus P.A. Karies zubov. Etiologiya, patogenez, epidemiologiya, klassifikatsiya: ucheb.-metod. posobie [Dental caries. Etiology, pathogenesis, epidemiology, classification: study guide]. Minsk, BGMU Publ., 2007, 35 p. (In Russian).
9. Yutskovskaya Ya.A., Koroleva A.Yu., Sergeenko A.E., Gapyak U.A. Razvitie nezhelatel'noy reaktsii posle primeneniya fillera na osnove gidroksiapatita kal'tsiya: klinicheskiy sluchay [Adverse reaction development after calcium hydroxyapatite-based filler application: a clinical case], Klinicheskaya dermatologiya i venerologiya [Clinical Dermatology and Venereology], 2022, vol. 21, no. 4, pp. 560-564. DOI: 10.17116/klinderma202221041560. (In Russian).
10. Gražulis S., Daškevič А., Merkys A. et al. Crystallography Open Database (COD): An open-access collection of crystal structures and platform for world-wide collaboration, Nucleic Acids Research, 2012, vol. 40, issue D1, pp. D420-D427. DOI: 10.1093/nar/gkr900.
11. Dudas C., Kutus B., Böszörmenyi E. et al. Calcium complexing behaviour of lactate in neutral to highly alkaline medium, Journal of Molecular Structure, 2019, vol. 1180, pp. 491-498. DOI: 10.1016/j.molstruc.2018.12.020.
12. Solonenko A.P., Golovanova O.A. Sintez, sostav i svojstva kompozitov na osnove fosfatov kal'ciya i zhelatina [Synthesis, composition, and properties of calcium phosphate-gelatin composites], Zhurnal neorganicheskoj khimii [Journal of Inorganic Chemistry], 2015, vol. 60, issue 9, pp. 1034-1040. DOI: 10.1134/S0036023615090181. (In Russian).
13. Tavoni M., Dapporto M., Tampieri A., Sprio S. Bioactive calcium phosphate-based composites for bone regeneration, Journal of Composites Science, 2021, vol. 5, issue 9, art.no. 227, 27 p. DOI: 10.3390/jcs509022.