Physical and chemical aspects of the study of clusters, nanostructures and nanomaterials
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Development of a manual extruder for liposome homogenization

I.E. Anufriev1, E.N. Muratova1, D.V. Korolev2, G.A. Shulmeister2, R.G. Valeev3, V.A. Moshnikov1

1 Saint Petersburg Electrotechnical University «LETI» named after V.I. Ulyanov (Lenin
2 Institute of Experimental Medicine, Almazov National Medical Research Centre , Ministry of Health of Russian Federation
3 Udmurt Federal Research Center of the Ural Branch of the Russian Academy of Sciences

DOI: 10.26456/pcascnn/2022.14.008

Original article

Abstract: Medications using liposomes are of great interest in pharmaceuticals. They increase the therapeutic index of the drug by enclosing the medicinal substance inside a biocompatible lipid envelope, which releases the solution only in the required area. Such drugs have already shown their effectiveness in the treatment of diseases related to oncology, dermatology, neurology, surgery, etc. To use liposomes for these purposes, it is necessary that their size be in the range from 50 to 200 nm. There are several ways to create vesicles of this size, but mostly they use either ultrasound exposure to a liposome solution or extrusion. The extrusion method is a method that allows to obtain the most homogeneous solution from liposomal particles. For extrusion, a special device – an extruder is required. It is a system that passes a liposomal solution under pressure through a filter with a certain pore size. In this paper, the process of liposome extrusion, types of liposomal extruders are considered and their pros and cons are evaluated, a model of a manual extruder capable of homogenizing up to 20 ml of solution was also developed. Different materials were considered and used for the construction of this device. The inspection of the extruder showed its operability and showed the advantages of using extrusion compared to the ultrasound exposure method.

Keywords: extrusion, liposomes, homogenization, membrane, porous aluminum oxide, extruder, 3D modeling

  • Ilya E. Anufriev – 5th year student, Department of Micro- and Nanoelectronics, Saint Petersburg Electrotechnical University «LETI» named after V.I. Ulyanov (Lenin
  • Ekaterina N. Muratova – Ph. D., Associate Professor, Department of Micro- and Nanoelectronics, Saint Petersburg Electrotechnical University «LETI» named after V.I. Ulyanov (Lenin
  • Dmitry V. Korolev – Dr. Sc., Associate Professor, Researcher, Research Laboratory of Nanotechnology, Centre for Experimental Biomodeling, Institute of Experimental Medicine, Almazov National Medical Research Centre , Ministry of Health of Russian Federation
  • Galina A. Shulmeister – Junior Researcher, Research Laboratory of Nanotechnology, Centre for Experimental Biomodeling, Institute of Experimental Medicine, Almazov National Medical Research Centre , Ministry of Health of Russian Federation
  • Rishat G. Valeev – Ph. D., Senior Researcher, Udmurt Federal Research Center of the Ural Branch of the Russian Academy of Sciences
  • Vyacheslav A. Moshnikov – Dr. Sc., Professor, Department of Micro- and Nanoelectronics, Saint Petersburg Electrotechnical University «LETI» named after V.I. Ulyanov (Lenin

Reference:

Anufriev, I.E. Development of a manual extruder for liposome homogenization / I.E. Anufriev, E.N. Muratova, D.V. Korolev, G.A. Shulmeister, R.G. Valeev, V.A. Moshnikov // Physical and chemical aspects of the study of clusters, nanostructures and nanomaterials. — 2022. — I. 14. — P. 8-16. DOI: 10.26456/pcascnn/2022.14.008. (In Russian).

Full article (in Russian): download PDF file

References:

1. Dmitrieva M.V., Lugen Bu, Oborotova N.A. et al. Metod ekstruzii v tekhnologii polucheniya liposom [Extrusion method in the technology preparation of liposomes], Vestnik Voronezhskogo gosudarstvennogo universiteta. Seriya: Khimiya. Biologiya. Farmatsiya [Proceedings of Voronezh State University. Series: Chemistry. Biology. Pharmacy], 2020, no. 3, pp. 87-96. (In Russian).
2. Svistelnik A.V., Khanin A.L. Liposomal'nye lekarstvennye preparaty: vozmozhnosti i perspektivy [Liposomal drugs: opportunities and prospects], Meditsina v Kuzbasse [Medicine in Kuzbass], 2014, vol. 13, no. 2, pp. 7-16. (In Russian).
3. Yuan F., Dellian M., Fukumura D.et al. Vascular permeability in a human tumor xenograft: molecular size dependence and cutoff size, Cancer Research, 1995, vol. 55, issue 17, pp. 3752-3756.
4. Blagbrough I.S., Zara C. Animal models for target diseases in gene therapy – using DNA and siRNA delivery strategies, Pharmaceutical Research, 2009, vol. 26, no. 1, pp. 1-18. DOI: 10.1007/s11095-008-9646-8.
5. Novikova A.A., Kezimana P., Stanishevskiy Ya.M. Metody polucheniya liposom, ispol'zuemykh v kachestve nositelej lekarstvennykh sredstv (obzor) [Methods of obtaining liposomes, used as drug delivery systems (review)], Razrabotka i registratsiya lekarstvennykh sredstv [Development and registration of medicines], 2017, no. 2 (19), pp. 134-138. (In Russian).
6. Mui B.L., Cullis P.R., Evans E.A., Madden T.D. Osmotic properties of large unilamellar vesicles prepared by extrusion, Biophysical Journal, 1993, vol. 64, issue 2, pp. 443-453. DOI: 10.1016/S0006-3495(93)81385-7.
7. Cho N.J., Hwang L.Y., Solandt J.J.R., Frank C.W. Comparison of extruded and sonicated vesicles for planar bilayer self-assembly, Мaterials, 2013, vol. 6, issue 8, pp. 3294-3308. DOI: 10.3390/ma6083294
8. Ong S.G.M., Chitneni M., Lee K.S., Ming L.C., Yuen K.H. Evaluation of extrusion technique for nanosizing liposomes, Pharmaceutics, 2016, vol. 8, issue 4, art. no. 36, 12 p. DOI: 10.3390/pharmaceutics8040036.
9. Rameez S., Bamba I., Palmer A.F. Large scale production of vesicles by hollow fiber extrusion: a novel method for generating polymersome encapsulated hemoglobin dispersions, Langmuir, 2010, vol. 26, issue 7, pp. 5279-5284. DOI: 10.1021/la9036343.
10. WHATMAN 6809-1112 Membrannyi shprits-fil'tr Anotop [Membrane Anotop syringe filter]. Available at: www.url: https://germeon.ru/ (accessed 05/27/2022).
11. Vybor materialov dlya 3D-pechati. Chast’ 1 [Selection of materials for 3D printing. Part 1]. 2013. Available at: www.url: https://3dtoday.ru/blogs/3dmindex/vybor-materialov-dlya-3d-pecati-cast-1 (accessed 27.05.2022).
12. Nanostructured semiconductors in porous alumina matrices: modeling, synthesis, and properties, ed. by R. Valeev, A. Vakhrushev, A. Fedotov, D. Petukhov. New York, Apple Academic Press, 2019, 280 p. DOI: 10.1201/9780429398148.
13. Muratova E.N., Luchinin V.V., Moshnikov V.A. et al., Features of the formation of nanoporous membranes based on alumina from foil and new fields of applications, Glass Physics and Chemistry, 2017, vol. 43, issue 2, pp. 163-169. DOI: 10.1134/S1087659617020122.

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